Wydanie 330(40)4 2016

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https://oa.zut.edu.pl/handle/20.500.12539/707

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Przeglądaj Wydanie 330(40)4 2016 wg Autor "Chojniak, Joanna"

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    PozycjaOpen Access
    Evaluation of toxicity of biologically synthesized silver nanoparticles (AG-NPs) using Lemna test and Algaltoxkit F
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Chojniak, Joanna; Drobniewska, Agata; Jałowiecki, Łukasz; Biedroń, Izabela; Płaza, Grażyna; Nałęcz-Jawecki, Grzegorz; Microbiology Unit, Institute for Ecology of Industrial Areas, Katowice; Department of Environmental Health Sciences, Medical University of Warsaw; Microbiology Unit, Institute for Ecology of Industrial Areas, Katowice; Microbiology Unit, Institute for Ecology of Industrial Areas, Katowice; Microbiology Unit, Institute for Ecology of Industrial Areas, Katowice; Department of Environmental Health Sciences, Medical University of Warsaw
    In the current study the toxicity of biologically synthesized Ag-NPs to Selenastrum capricomutum and Lemna minor was assessed. The supernatants from the Bacillus subtilis cultures were applied for the Ag-NPs synthesis. The following media were used for culturing of Bacillus subtillis (I’-1a): molasses (Mol.), brewery effluent (# 6) and Luria-Bertani (LB) medium. Silver nitrate was added to the culture supernatants to the final concentration of 1 mM. The Ag-NPs presence was monitored by UV-Vis spectrophotometer. Toxicity tests were made according to OECD Guideline 201 (2006) and ISO standard 20079 (2006). In the test AlgaltoxkitF the two parameters cell growth and content of chlorophyll a were evaluated. However, in the test with Lemna minor the five endpoints including: number and total area of fronds, fresh weight (f.w.), chlorophylls a (Chl a) and b (Chl b) were used to assess phytotoxicity. Ag+ ions were considered to be the most toxic for Selenastrum capricornutum and Lemna minor than Ag-NPs biologically synthesized. The toxicity of biologically synthesized Ag- NPs was lower to S. capricomutum and to L. minor for all analyzed parameters compared to the toxicity of Ag+. However, the toxicity of Ag-NPs synthesized in the supernatant from the culture of Bacillus subtilis growing on brewery waste was higher compared to the Ag-NPs synthesized in the supernatants from the cultures of strain growing on molasses and LB media. The variability in sensitivity of both organisms towards Ag-NPs was observed.