Przeglądaj wg Autor "Chmiel, Maria J."

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  • Miniatura
    PozycjaOpen Access
    The assessment of water sanitary quality and the presence of drug-resistant Escherichia coli strains in waters used for recreation in lesser Poland
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Chmiel, Maria J.; Wójtowicz, Paulina; Łojas, Ewelina; Department of Microbiology, University of Agriculture, Kraków; Department of Microbiology, University of Agriculture, Kraków; Department of Microbiology, University of Agriculture, Kraków
    The presence of multi-drug resistant bacteria is no longer only a clinical problem because increasingly, during the microbiological water and soil analysis, the antibiotic resistant bacteria are isolated, which can be a significant health hazard. The aim of this study was the assessment of water sanitary quality and the occurrence of drug resistant strains of Escherichia coli in 10 sampling points located on selected water reservoirs used for recreation on the Dunajec river and reservoirs in Krakow area and Kryspinow. Collected water samples were examined by the membrane filtration technique to assess the number of E. coli. Drug resistance was tested using the disc diffusion method as well as the ESBL assay according to KORLD and EUCAST standards. Based on the results, it was found that in two sampling points located on the Biały Dunajec river the population of E. coli exceeded standards intended for bathing. Resistant strains were isolated mainly in one bathing place - Kryspinow – Na Piaskach, but they were also present in the waters of the Dunajec river. Most often isolates were resistant only to one of the tested antibiotics, the multidrug resistant E. coli were no isolated. The tested strains were mainly resistant to ticarcillin, sulfamethoxazole/trimethoprim and tetracycline. All the tested strains were ESBL negative.
  • Miniatura
    PozycjaOpen Access
    Bacteriological contamination of water in selected bathing areas in Małopolska
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Chmiel, Maria J.; Mazur, Edyta; Król, Teresa; Department of Microbiology, University of Agriculture, Kraków; Department of Microbiology, University of Agriculture, Kraków; Department of Microbiology, University of Agriculture, Kraków
    Microbiological contamination of waters used for recreational purposes may constitute a serious danger to the health of users. Only a few reservoirs in Lesser Poland are subjects of constant control and meet the microbiological criteria. The work was aimed at evaluating the degree of microbiological contamination of selected bathing areas in Małopolska voivodeship in the municipal areas Tarnow and Krakow. Samples were taken from 10 sampling points, three times in the period from June to October 2016. Microbiological analyzes of water were performed using membrane filtration and serial dilutions methods. The total number of mesophilic and psychrophilic bacteria as well as number of Escherichia coli, fecal streptococci (enterococci) and staphylococci were determined. In all examined reservoirs the occurrence of microbiological indicators of bad hygienic condition of water was confirmed. Numerous occurred bacteria of the species E. coli, which was the main reason for the water being taken to those that did not meet the bathing area requirements. More contaminated were bathings in the municipality of Kraków than Tarnów, but these were not statistically significant differences. The amount of psychrophilic bacteria in the waters was higher than mesophilic ones, suggesting that the main source of microorganisms is the natural environment. Recreational areas under constant monitoring were characterized by slightly less contamination, but rarely met criteria for bathing due to the large number of indicator bacteria. The biggest water pollution was found at the peak of the tourist season in August. Considering the average values of measurements only bathing “Katamaran” meet the requirements.
  • Miniatura
    PozycjaOpen Access
    Evaluation of the quality of surface water in the vicinity of waste water treatment plants based on bacteriological contamination
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Chmiel, Maria J.; Lis, Ewelina; Korta-Pepłowska, Magdalena; Department of Microbiology, University of Agriculture in Kraków; Department of Microbiology, University of Agriculture in Kraków; Department of Microbiology, University of Agriculture in Kraków
    Although the extension of canalization and the building of numerous sewage treatment plants in southern Poland significantly affected the water quality by eliminating the constant and uncontrolled inflow of pollutants from individual farms, it is also the same treatment that can cause local deterioration of the watercourses sanitary state in sections after the discharge of water from the treatment plant. The aim of this study was to evaluate the effect of effluent discharged from sewage treatment on the microbiological quality and the presence of Escherichia coli in the waters of the rivers. Sampling points were located upstream and downstream of discharge on 6 rivers: Wisła, Dłubnia i Chechło, Minóżka, Krzeszówka and Prądnik which are receivers of effluent from the local wastewater treatments. In the tested watercourses the number of mesophilic and psychrophilic bacteria was determined by serial dilution method as well as the presence of Escherichia coli by membrane filtration method. The results show that the flow of wastewater greatly increases the number of bacteria in the water which indicates that the purification of waste water does not eliminate all of the microbial contaminants. In water samples from the sampling points located by sewage the presence of numerous E. coli was also confirmed. The size of wastewater and the volume of the river had an impact on the degree of microbiological water pollution. The analysis indicate the need for continuous monitoring of plant operation and control of the bacteriological quality of wastewater discharged into surface waters.