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Pozycja Open Access The Effect of Mannitol and Sorbitol on Soybean In Vitro Development(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Kulpa, Danuta; Gawlik, Andrzej; Matuszak-Slamani, Renata; Włodarczyk, Małgorzata; Bejger, Romualda; Sienkiewicz, Mariola; Gołębiowska, Dorota; Semeniuk, Anna; Department of Plant Genetic, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Genetic, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, PolandThe aim of this study was to examine an effect of osmotic stress, induced by mannitol (Mn) and sorbitol (So) in concentrations (0–200 mM) on seed germination and development of common soybean (Glycine max L.) seedlings in in vitro conditions. The analysis of the effectiveness of the porcession was made by assessing the length of the stems and roots as well as the fresh and dry mass of 3-week-old seedlings. The biometric index values differed depending on the type of substance used. While Mn at the lowest concentration (50 mM) did not affect the biometric parameters studied, So (50 mM) stimulated the growth of seedlings, root elongation and the number of leaves and fresh weight of plants. The unfavorable effect on biomass was noticed at higher concentrations of both substances. Mn (> 50 mM – <150 mM) negatively influenced the fresh and dry content of the seedlings. In turn, Mn (150 and 200 mM) also caused growth inhibition, decreased number and length of leaves. So (200 mM) significantly inhibited the growth of seedlings, reducing the dry matter.