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Pozycja Open Access Blood Plasma Progesterone Levels do not Correlate with Litter Size in the American Mink (Neovison vison)(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Seremak, Beata; Dworecka-Borczyk, Marta; Felska-Błaszczyk, Lidia; Lasota, Bogdan; Department of Biotechnology of Animal Reproduction and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, Poland; Department of Biotechnology of Animal Reproduction and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, Poland; Laboratory of Animal Anatomy Faculty of Biotechnology and Animal Breeding, West Pomeranian University of Technology, Szczecin, Poland; Department of Biotechnology of Animal Reproduction and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, PolandThe aim of the study was to test whether there is a relationship between the plasma concentration of progesterone in a given stage of pregnancy and the litter size at birth. The study was carried out on a mink farm located in western Poland. Blood samples for analyses were drawn from year-old White Hedlund (WH) females. Samples were collected from 30 females, first on 22 February, i.e. before the mating season, and on 12 April, from pregnant females, about 3 weeks before expected parturitions. Plasma progesterone levels revealed a high individual variability within the studied group of mink. No significant correlation was found between blood plasma progesterone in pregant females and the average litters size at birth.Pozycja Open Access Estriol - Alternative Pregnancy Diagnosis Marker in the Mink?(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2013) Skuratko, Agata; Lasota, Bogdan; Felska-Błaszczyk, Lidia; Department of Biotechnology of Animal Reproduction and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, Poland; Department of Biotechnology of Animal Reproduction and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, Poland; Department of Animal Anatomy, West Pomeranian University of Technology, Szczecin, PolandEndogenne estrogeny, do których zalicza się estradiol, estron i estriol, to niejednorodna grupa żeńskich hormonów płciowych. Estriol jest hormonem steroidowym występującym w organizmie kobiety w małych ilościach. Rola estriolu w czasie ciąży jest bardzo ważna, ponieważ jego synteza zwiększa się w tym okresie i stanowi 90% wszystkich estrogenów. W warunkach prawidłowych produkcja estriolu wzrasta wraz z rozwojem płodu, a stężenie hormonu w trzecim trymestrze ciąży wzrasta trzykrotnie. Utrzymujące się niskie lub gwałtownie zmniejszające się stężenie estriolu sugeruje stan zagrożenia płodu. Użycie testu do pomiarów tego estrogenu w ślinie może pomóc we wczesnym wykryciu ryzyka porodu przedwczesnego. Ze względu na odmienny typ łożyska charakter ludzkiego metabolizmu estriolu może znacznie różnić się od metabolizmu samic norki. Ze względu na wyraźny wzrost stężenia estriolu dopiero w 2. połowie ciąży oraz ze względu na występowanie diapauzy jego atrakcyjność jako markera wczesnej ciąży dla hodowcy norek się zmniejsza. Jednakże warte rozważenia jest jego zastosowanie do monitorowania przebiegu ciąży, szczególnie w przypadku możliwości oznaczenia koncentracji w ślinie.Pozycja Open Access Human chorionic gonadotropin (hCG) applied instead of first mating improves reproduction in mink (Neovison vison)(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Seremak, Beata; Pławski, Kamil; Felska-Błaszczyk, Lidia; Lasota, Bogdan; Department of Biotechnology of Animal Reproduction and Environmental Hygiene West Pomeranian University of Technology, Szczecin; Department of Biotechnology of Animal Reproduction and Environmental Hygiene West Pomeranian University of Technology, Szczecin; Laboratory of Animal Anatomy, West Pomeranian University of Technology, Szczecin; Department of Biotechnology of Animal Reproduction and Environmental Hygiene West Pomeranian University of Technology, SzczecinThe aim of the study was to determine how an administration of chorionic gonadotropin (hCG) in place of the first mating would affect breeding performance of female American mink (Neovison vison). The experiment was conducted in two consecutive breeding seasons on groups of females composed of one- and two-year old mink, similar in body weight, belonging to three colour morphs: Sapphire, Standard Brown (Wild), and Mahogany. The experimental group were females treated with an injection of 20 IU hCG instead of being subjected to the first mating. The females were first mated on day 8 after the treatment, repeated on the following day (scheme: 8 + 9). The control females were mated in a conventional way, three times (scheme: 1 + 8 + 9). Statistically significant differences were found in litter sizes (both numbers of born and live-born kits) in favour of the hormonally treated females. The Mahogany females, treated with hCG, produced larger litters in both studied seasons, whereas Standard Brown and Sapphire females – in the second year of the experiment. The results indicate a positive effect of hCG stimulation on the fertility of female mink and advocate its use in the breeding practice.Pozycja Open Access Oxygraphic evaluation of activity of respiratory enzymes in boar's semen(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Kopczyński, Piotr; Lasota, Bogdan; Ogoński, Tadeusz; Stankiewicz, Tomasz; Gaczarzewicz, Dariusz; Skolmowska, Magdalena; Department of Physiological Chemistry, West Pomeranian University of Technology, Szczecin; Department of Biotechnology of Animal Reproduction and Environmental Hygiene, West Pomeranian University of Technology, Szczecin; Department of Physiological Chemistry, West Pomeranian University of Technology, Szczecin; Department of Physiological Chemistry, West Pomeranian University of Technology, Szczecin; Department of Biotechnology of Animal Reproduction and Environmental Hygiene, West Pomeranian University of Technology, Szczecin; Department of Physiological Chemistry, West Pomeranian University of Technology, SzczecinIn the article advantages of the use of oxygraphic method for metabolic examination of mitochondrial respiratory enzymes in boar spermatozoa were described. Investigations were performed on semen samples taken from 36 boars of 4 breeds, at the age from 8 to 40 months. All samples of semen characterized at least 80% content of motile spermatozoa. The rate of oxygen uptake was measured with the use of Clark type electrode connected with analog-digital recorder. Measurements were executed in temperature 37°C, and the rate of oxygen uptake (v) was expressed in nmol O2 · min–1 per 108 spermatozoa. The values of oxygen consumption rate after application of succinate (vs) and PMS (vp) made up the basis of analysis. The results show that the method of oxygraphic evaluation of spermatozoa movement system described in the paper makes possible the precise investigation of the spermatozoa respiratory chain status and enables selection of boars with the best parameters of semen. This method can be taken in consideration at semen examination in the course of boar's selection for the AI stations, after boar's reproductive disease, in periodical semen tests and also when taking decision about the boar's elimination.Pozycja Open Access Population of Tigers in Polish Zoos in 2000–2006(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2015) Lasota, Bogdan; Ronowska, Anna; Department of Reproductive Biotechnology and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, Poland; Department of Reproductive Biotechnology and Environmental Hygiene, West Pomeranian University of Technology, Szczecin, PolandCurrently in the natural environment tigers live in areas of eastern Turkey, through southern Asia to southeastern Siberia, Malay Peninsula and Sumatra, Java and Bali. Generally, the area of occurrence of these predators declined and some subspecies extincted, for example Javanese tiger, Balinese tiger, the Caspian tiger. Zoos are therefore still places where you can see live the big cats. They are also held in Poland’s zoos, where increasingly various programs are carried out for breeding and reproduction of this endangered species. The aim of this study was to analyze the population of tigers and selected indicators of reproduction and mortality in 2000–2006. On the base of data from the Handbook of Zoos, which contents the information about population of animals in Polish zoos, and from cards of animals the analysis of population of Amur tigers, Bengal, Siberian and White tigers in Polish zoos were carried out. The analysis included also some reproductive indicators. Polish population of tigers in the analyzed years decreased by approx. 45%. This decrease was affected to a greater extent females. Total loss was not compensated by the number of born and rearing offspring. To maintain the number of tigers in Polish zoos require intensive efforts to increase the reproduction of this species, and the purchase or "rent" females with proven reproductive capacity.