Przeglądaj wg Autor "Melnyk, Oleg"

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  • Miniatura
    PozycjaOpen Access
    Analysis of Production and Reproduction Indicator of Polish Holstein-Friesian Cattle Oin Three Consecutive Lactations.
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2022) Stefaniak, Wiktoria P.; Czerniawska-Piątkowska, Ewa; Kowalewska-Łuczak, Inga; Kostiuk, Volodymir; Melnyk, Oleg; Mylostyvyi, Roman; Wrzecińska, Marcjanna; Kossakowski, Kamil; Student Scientific Association of Breeders of Ruminant Animals “TAURUS” at the Department of Ruminant Science, West Pomeranian University of Technology in Szczecin, Poland; Student Students Scientific Association of Animal Geneticists, Department of Genetics and General Animal Breeding, West Pomeranian University of Technology in Szczecin, Poland; Department of Ruminant Science, West Pomeranian University of Technology in Szczecin, Poland; Department of Genetics and Animal Breeding, West Pomeranian University of Technology Szczecin, Poland; National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine; National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine; Department of Technology Processing of Livestock Products, Dnipro State Agrarian and Economic University, Dnipro, Ukraine; Department of Ruminant Science, West Pomeranian University of Technology in Szczecin, Poland; Wrocław University of Environmental and Life Sciences, Department of Environment Hygiene and Animal Welfare, Wrocław, Poland
    The aim of the research was to determine the productivity and reproduction indices of the Polish Holstein-Friesian Black-and-White variety cows in three consecutive lactations. The research was carried out in the Experimental Station of the National Research Institute Kołbacz on the Dębina farm. The herd of 1025 cows were kept in free-stall barns and fed with TMR system. The yield (kg) of milk, FCM milk, protein and fat was analyzed; content (%) of protein and fat and selected reproductive parameters such as age at the first calving, as well as inter-pregnancy periods and calving interval, pe riod service, postpartum downtime in three consecutive 305-day lactations. The studies indicated that the average yield of milk, fat and protein showed an increasing tendency in the next three lactations. The average protein and fat content were similar. The highest results of these ingredients were ob tained in cows in the second lactation. Lactation efficiency influenced the value of the analyzed fertility indices. With the increase in cows’ productivity, the calving interval and inter-pregnancy period, includ ing period service and postpartum downtime, were significantly longer. The longest calving interval and inter-pregnancy period were observed in the third lactation, and the shortest in the first lactation of cows. With the extended of the calving period, the yield of milk, protein, and fat increased. The age at the first calving was the lowest in the 2nd and the highest in the 3rd lactation. The cows calving first for the first time had the highest milk fat content and the shortest calving period. The service period was the longest in the 2nd lactation and occurred in cows with the highest fat content. Postpartum downtime in the studied herd of cows was the longest in the third lactation. The resting period was extended with the increase in the yield of: milk, FCM milk, protein and fat.
  • Miniatura
    PozycjaOpen Access
    Morphological Changes in the Tissues of the Rabbit Knee Joint Due to Experimental Osteoarthritis After the Use of Mesenchymal Stem Cells
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2021) Malyuk, Nikolai; Demiantseva, Yuliia; Kharkevych, Yuriy; Bokotko, Roman; Melnyk, Oleg; Gryzinska, Magdalena; Department of Surgery and Pathophysiology named after acad. I.O. Povazhenko, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine; Department of Surgery and Pathophysiology named after acad. I.O. Povazhenko, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine; Department of Surgery and Pathophysiology named after acad. I.O. Povazhenko, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine; Department of Surgery and Pathophysiology named after acad. I.O. Povazhenko, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine; Department of Animal Anatomy, Histology and Pathomorphology named after academician Vladimir G. Kas’janenko, National University of Life and Environmental Sciences of Ukraine, Kyiv, Ukraine; Institute of Biological Basis of Animal Production, Faculty of Biology, Animal Sciences and Bioeconomy, University of Life Sciences in Lublin, Poland
    The purpose of the study was to investigate the regenerative processes in the knee joint of rabbits with experimental osteoarthritis after using of allogeneic bone marrow stem cells and a traditional treatment with the non-steroidal anti-inflammatory drug Meloxicam. For the experiment were used 27 male California rabbits (males). Three groups of animals were formed: a control group; the first experimental group treated by the traditional method; the second experimental group treated with allogeneic mesenchymal stem cells (MSC). Animals in the three groups were subjected to osteoarthritis of the knee joint by double injection of 3.44% retinol acetate into the joint cavity at a dose of 1 ml at intervals of 7 days. Tissue from the affected site was sampled for histological examination at 7, 14 and 28 days. The histological sections were stained with haematoxylin-eosin and examined under a microscope. It has been established that intra-articular administration of 3.5 × 106 cells of allogeneic MSCs in experimental osteoarthritis contributes to the restoration of the superficial layer of cartilage, as evidenced by the formation of columns of chondrocytes in the middle layer of articular cartilage and the appearance of isogenic groups of cartilage cells with basophilic cytoplasm in the matrix, uniform articular surface. The use of the traditional method of treating rabbits using the drug Meloxicam is accompanied by incomplete chondrogenesis: part of the chondrocytes is localized in typical chambers, in some cases chondrocyte chambers did not differentiate; articular cartilage had unequal thickness, cell placement was uneven.