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Pozycja Open Access Alleviating Effects of Ascorbic Acid on Lead Toxicity in Goji (Lycium barbarum L.) in Vitro(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Krupa-Małkiewicz, Marcelina; Kruczek, Arleta; Pelc, Justyna; Smolik, Beata; Ochmian, Ireneusz; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland; Department of Horticulture, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Horticulture, West Pomeranian University of Technology, Szczecin, PolandLead (Pb) is the most common heavy metal pollutant in the environment. The objective of the presented study was to investigate the ameliorative effect of exogenous 1 mM ASA on key growth and biochemical parameters in Lycium barbarum seedlings under heavy metal (Pb(NO3)2) stress in vitro. Nodal cutting with an axillaries bud were used as an explants. The results showed that lead accumulation in goji explants had negative influence on morphological parameters of plant growth, such as shoot and root length. Lead caused a significant reduction in chlorophylls and carotenoid content, increased lipid peroxidation and induced significant accumulation of proline in goji leaves. Addition to MS medium 1 mM ASA greatly alleviated Pb-induced growth inhibition and Pb-induced MDA and proline accumulation. Presence of ASA in the MS medium under heavy metal stress increased plant fresh and dry mass with no significant effect on plant water content.Pozycja Open Access Effect of Crop Variety and Covering Plants on Formation of Biochemical Parameters of Chinese Cabbage (Brassica Chinensis Juslen.)(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2015) Bychowiec, Katarzyna; Smolik, Beata; Słodkowski, Paweł; Auriga, Alicja; Pelc, Justyna; Sędzik, Maja; Department of Horticulture, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Horticulture, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, PolandThe aim of the study was to investigate the effect of covers (foil perforated by 100 and 400 holes per 1 m2 and woven polypropylene Argyle P17) and method of cultivation (sowing seed and a base solution) on selected physiological parameters (concentration of carbon dioxide and transpiration) and bio-chemical (concentration of proline and malondialdehyde) in the leaves of two varieties of Chinese cabbage (Yoj-Choy and Green Fortune F1) growing in the field. Cover used in the experiment affected the biochemical parameters studied plants. The highest uptake assimilation was demonstrated in the case of perforated foil 400 holes per 1 m2, whereas the smallest in the case of non-woven polypropylene. The foil also consed the highest level of transpiration. Woven to the greatest extent caused the increase the concentration of proline and malondialdehyde in leaves of cabbage. The highest concentrations of these parameters were found in a Yoi-Choy F1 variety.Pozycja Open Access Effect of sodium fluoride on some morphological and physiological parameters of 10-day-old seedlings of various plant species(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Pelc, Justyna; Smolik, Beata; Krupa-Małkiewicz, Marcelina; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, SzczecinFluorine is one of the most toxic elements for plants resulting from its high electronegativity. Fluorine compounds, the penetration into the plant tissue, may cause a variety of physiological and biochemical changes. The aim of this study was to determine the influence of 10 mM sodium chloride (NaF) on morphological parameters (root length, shoot length) and physiological parameters (proline, total chlorophyll and carotenoids) in leaves of 10-day-old seedlings of various species of crop plants under laboratory conditions. Results obtained in this experiment showed that fluoride adversely affecting the morphological parameters and physiological parameters of the test plants when compared to control. Among 10 studied plants, three species (barley, radish ‘Minowase Summer Cross’, lupine) indicated high tolerance to NaF compared to the other tested plants. The most sensitive to lead exposure were wheat, radish ‘Carmen’, alfalfa, sunflower, tomato.Pozycja Open Access Genotypic Differences between Tomato Cultivars Differing in Their Response to Salinity Stress(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2014) Krupa-Małkiewicz, Marcelina; Franczak, Małgorzata; Grabiec, Marta; Smolik, Beata; Smolik, Miłosz; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Biology, Masaryk University, Brno, Czech Republic; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, PolandCelem pracy było określenie różnic genotypowych między trzema odmianami uprawnymi pomidora [Lycopersicon esculentum (Mill.)] – Zorza, Paw i Złoty Ożarowski, poddanych działaniu stresu solnego w warunkach laboratoryjnych. Tolerancję na zasolenie określono za pomocą testu szalkowego na płytkach Petriego, stosując trzy stężenia soli: 25 mM · dm–3 NaCl + 25 mM · dm–3 KCl, 25 mM · dm–3 NaCl + 50mM · dm–3 KCl oraz 50 mM · dm–3 NaCl. Kontrolę w doświadczeniu stanowiła woda sterylna. Na podstawie przeprowadzonych pomiarów biometrycznych (zdolność kiełkowania, długość siewki i korzeni), badane genotypy podzielono na trzy klasy fenotypowe według ich cech morfologicznych. Siewki uznane za tolerancyjne przydzielono do pierwszej klasy fenotypowej, średniotolerancyjne do drugiej, a wrażliwe do klasy trzeciej. Różnice genotypowe pomiędzy badanymi obiektami określono, stosując technikę ISSR-PCR. Zaobserwowano różnice w długości amplifikowanych sekwencji miedzymikrosatelitarnych pomiędzy badanymi odmiana pomidora, jak i siewkami należącymi do różnych klas fenotypowych. Podobieństwo genetyczne między odmianą Paw i Złoty Ożarowski wynosiło 45,2%, a między odmianą Zorza i Paw – 54,8%.Pozycja Open Access Interactive effects of salinity stress with or without nicotinamide on physiological and biochemical parameters of tomato seedling(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Krupa-Małkiewicz, Marcelina; Ostojski, Dominik; Sędzik, Maja; Pelc, Justyna; Smolik, Beata; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, SzczecinThe aim of this study is to determine the effect of both NaCl and KCl salt alone or in combination with nicotinamide, on growth and some biochemical parameters of Vilma cultivar of tomato under laboratory and field conditions. The combinations of salt solutions used had a negative impact on the ability of seed germination and morphological characteristics of 14-day-old tomato seedlings. The addition of NaCl salt had a positive impact on the content of Chl a and Car in contrast KCl salt solution led to the decrease of photosynthetic and non- -photosynthetic pigments. Under field conditions, NaCl salt solution exhibited inhibitory effect on plant growth, concentration of Chl a, Chl b, and Car, simultaneously increasing oxidative stress parameters (proline and malondialdehyde – MDA). Moreover, leaves of tomato from the control group were darker in comparison to the remaining plants. It was observed that the addition of nicotinamide to the solution did not show protective effect on plants grown under salinity, except in a small increase of the concentration of proline.Pozycja Open Access Reakcja wybranych odmian żyta ozimego na stres wywołany różnymi czynnikami abiotycznymi(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2012) Smolik, Beata; Zakład Biochemii, Zachodniopomorski Uniwersytet Technologiczny w SzczecinieThe pot experiment was conducted in laboratory conditions. The soil used in experiment was loamy sand with 1.2% of organic carbon. To individual soil samples the following solutions were added 2.5 mM Pb(NO3)2 (207 mg Pb+2), 30 mM NaF (570.0 F– ), 0.05 mM H2SeO3 (3,95 Se4+), aqueous emulsion of Izoturon 500 SC herbicide in an amount of 6.5 mm3 · kg–1 soil. The aim of this study was to determine the influence of chosen pollutants on biometric parameters and SOD activity in three winter rye cultivars. Substances applied to the soil significantly reduced growth, fresh weight and superoxide dismutase activity in three winter rye cultivars planted in contaminated soil in comparison with control plants. Rye cultivars were characterized by different tolerance to chosen substances. It was found that cultivar ‘Chrobre’ was more sensitive to introduced pollutants than ‘Skat’ and ‘Dańkowskie Diamant’.