Przeglądaj wg Autor "Sobczyk, Martyna"

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    Dart markers linked with genes controlling restoration of male fertility in hybrid rye cultivars with improved pollen shedding
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Stojałowski, Stefan; Hanek, Monika; Orłowska, Marta Orłowska; Sobczyk, Martyna; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin
    In this study, we aimed to apply a high-throughput genotyping method to map genes important for the restoration of male fertility in a hybrid cultivar of rye containing the Pampa sterilizing cytoplasm. The diversity arrays technology (DArT) was used to analyse 48 individuals of the F2 population obtained by crossing the male-sterile S305P line with a plant randomly chosen in the Gonello F1 cultivar. In addition to DArT markers, a set of previously published PCR-based markers was also used for genotyping. In total, more than 3300 markers were used in this study. A mapping analysis allowed a construction of seven linkage groups containing 763 markers covering a total distance of approximately 520 cM. Eighty molecular markers were applied to identify genomic regions important for the male fertility restoration. Their distribution indicated the presence of a major and minor restorer genes on chromosomes 4RL and 1R, respectively. These results were consistent with previous reports on the genetic control of the male fertility in the CMS-Pampa. Moreover, new molecular markers located in chromosomal regions significantly associated with the restoration of male fertility were found as well, including PCR-based markers converted from the DArT markers.