Przeglądaj wg Autor "Szablicka, Dorota"

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    PozycjaOpen Access
    The Incidence of Spermatozoa with Morphological Changes in Semen Collected from Canine Epididymides
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2020) Wysokińska, Anna; Chłopik, Angelika; Szablicka, Dorota; Siedlce University of Natural Sciences and Humanities, Faculty of Agrobioengineering and Animal Husbandry, Siedlce, Poland; Siedlce University of Natural Sciences and Humanities, Faculty of Agrobioengineering and Animal Husbandry, Siedlce, Poland; Siedlce University of Natural Sciences and Humanities, Faculty of Agrobioengineering and Animal Husbandry, Siedlce, Poland
    The aim of the study was to analyze the incidence of spermatozoa with morphological changes in semen collected from canine epididymides. The study was conducted on five dogs with gonads obtained from them as a result of routine castration performed at a veterinary clinic. Epididymides were isolated after gonadal lavage with PBS fluid. Semen was collected by incising individual parts of the epididymis (caput, corpus, cauda) with a sterile scalpel, when placed in separate Petri dishes. Sperm morphology was prepared from the semen collected in this way. The preparations were stained with the SpermBlue method. The sperm morphology was assessed using a 100x immersion lens with a Nikon E-50i light microscope. In each preparation, the morphological structure of 200 spermatozoa was assessed, specifying the sperm cells with normal morphological structure and those with changes in their head, tail, and those with cytoplasmic droplets. Based on the performed study, it was found that there are differences in the incidence of sperm cells with morphological changes in individual sections of the epididymal duct. The largest share of spermatozoa with normal morphological structure was observed within the corpus of the epididymis. The most spermatozoa with head changes and with cytoplasmic droplets were found in semen collected from the epididymal caput. There were more than 14% more spermatozoa with head changes located in the epididymal caput than in the epididymal cauda (P ≤ 0.05). The semen collected from the cauda of the epididymis showed significantly more sperm cells with tail changes than in the remaining parts of the epididymis. As semen moved through the individual segments of the epididymis, the proportion of spermatozoa with head changes and cytoplasmic droplets decreased, while the proportion of spermatozoa with tail changes increased.