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Pozycja Open Access The effect of water activity and temperature on the growth and lipolytic activity of Penicillium chrysogenum (Thom) and Eurotium herbariorum (Wigg.) strains(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Janda, Katarzyna; Markowska-Szczupak, Agata; Ulfig, Krzysztof; Department of Biochemistry and Human Nutrition, Pomeranian Medical University in Szczecin; Institute of Inorganic Technology and Environment Engineering, West Pomeranian University of Technology, Szczecin; School of Public Health, Medical University of Silesia, BytomThe aim of the study was to determine the effect of water activity (aw) and temperature on the growth and tributyrin hydrolysis of Penicillium chrysogenum and Eurotium herbariorum strains isolated from rape, soya-bean and sunflower seeds. The study demonstrated differences in daily growth rates, tributyrin hydrolysis zones and tributyrin hydrolysis indices in different strains of the same species; depending on the seeds they had been isolated from. E. herbariorum strains, unlike P. chrysogenum strains, did not grow on tributyrin at 15°C and 0.850 aw. No hydrolysis zones of oil substrate were found under these conditions. E. herbariorum and P. chrysogenum showed marked differences in their tributyrin hydrolysis indices. For P. chrysogenum strains somewhat higher values were recorded for cultures at 15°C. However, the highest indices of tributyrin hydrolysis at 15°C and 25°C were recorded at the lowest level of aw 0.850.