Wydanie 351(52)4 2019

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https://oa.zut.edu.pl/handle/20.500.12539/691

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Przeglądaj Wydanie 351(52)4 2019 wg Temat "fermentacja in vitro"

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    Methanogenesis and Synthesis of Volatile Fatty Acids in the Rumen of Cows and Their Changeability Under the Influence of Ensiled Plants Additives
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2019) Mikołajczyk, Katarzyna; Pecka-Kiełb, Ewa; Zachwieja, Andrzej; Department of Biostructure and Animal Physiology, Wroclaw University of Environmental and Life Sciences; Department of Biostructure and Animal Physiology, Wroclaw University of Environmental and Life Sciences; Department of Cattle Breeding and Milk Production, Wroclaw University of Environmental and Life Sciences
    The aim of the research was determining the influence of select silages used in cow breeding on the production of methane and volatile fatty acids in the rumen digesta in the in vitro study. The rumen digesta for the experiment were obtained from 10 Polish Holstein-Frisian cows of black and white variety with a probe. The sampled digesta were then diluted in buffer solution and homogenized. To prepare fermentation samples, the digesta were placed in serum bottles with 1 g of supplementary substrates: I – corn silage, II – grass silage. The bottles were then flushed in CO2 and in vitro fermentation was performed at 39oC for 8 and 24 h. After the fermentation, the amount of fermentation gas was measured and the general concentration and profile of VFA were determined using a gas chromatograph (Agilent Technologies 7890A GC System). The research determined the influence of corn and grass silages on the production of methane in the cow rumen, the synthesis of VFA and the changeability of particular VFA fraction shares in the process of in vitro fermentation. The use of grass silage decreases methane production in the fermentation of rumen digesta. Corn silage used as a substrate in the fermentation of ruminal fluid increases propionate and butyrate production. The obtained results suggest that the use of grass silage reduces methane production in the rumen digesta of cows, but because of changes in the proportion of propionate and butyrate it is necessary to supplement the cow feed with grass silage as well.