Wydanie 338(44)4 2017

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https://oa.zut.edu.pl/handle/20.500.12539/701

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    PozycjaOpen Access
    Biological control of Fusarium culmorum isolated from the cultivation of white asparagus by Pseudomonas fluorescens
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Grata, Katarzyna; Department of Biotechnology and Molecular Biology, Opole University
    Assessment of the effect of Pseudomonas fluorescens I-4 (Ps I-4) on the Fusarium culmorum isolated from the cultivation of white asparagus was the aim of the conducted research. Fusarium culmorum and other fungi were isolated on the PDA and asparagus media. Among the isolates obtained from soil, F. oxysporum and F. culmorum were the most dominant pathogens. The biological properties of non-volatile and volatile compounds of Ps I-4 were assessed with the culture-plate method on the PDA and Czapek media. The fungistatic activity of bacterium was determined against the growth rate index, the spore germination index and the rate of mycelial growth and spore inhibition. The results show differences in the fungistatic activity of P. fluorescens, dependent on the age and density of the bacterial culture, the type of medium and the presence of volatile or non-volatile metabolites. The obtained results prove, that Ps I-4 show a higher activity against the fungal spores germination (82–94% inhibition) than on mycelium growth of F. culmorum. The highest inhibition of the linear growth of mycelium has been observed for the supernatants obtained from a 6-hour culture (64–76% inhibition). P. fluorescens I-4 showed positive reactions for the production of HCN, siderophores, protease, lipase and phosphate solubilisation.