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Pozycja Open Access Waste from the Harvesting of Button Mushroom (Agaricus bisporus) as a Source of Natural Melanin(Wydawnictwo Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Łopusiewicz, Łukasz; Center of Bioimmobilisation and Innovative Packaging Materials, West Pomeranian University of Technology, Szczecin, PolandNatural melanins are of great potential value and application in the fields of pharmacology, cosmetics and functional foods. In the present study, natural melanin was reclaimed from waste after the harvesting of button mushroom (Agaricus bisporus). The physiochemical properties of raw and purified melanin were determined, including their ultraviolet-visible (UV-Vis), infrared (IR) and Raman spectra. Colour values, polyphenolic content, antioxidant and antibacterial activity were also evaluated. The result showed that the characteristics of the obtained pigments were similar to synthetic L-DOPA melanin. Raw and purified melanins showed differences in chemical composition, antioxidant activity and light barrier properties. Isolated melanins showed antibacterial activity against Enterococcus faecalis and Pseudomonas aeruginosa but were inactive against Bacillus cereus, Escherichia coli and Staphylococcus aureus. This is the first research on the character of melanin reclaimed from waste after the harvesting of A. bisporus.