Zastosowanie metod RAPD i ISSR do oceny mieszańców pszenżyta z Aegilops crassa 4x Boiss
Ładowanie...
Data
2010
Tytuł czasopisma
ISSN czasopisma
Tytuł tomu
Wydawca
Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie
Abstrakt
In the study the genetic similarity in triticale (6x) hybrids with Aegilops crassa (4x)
Boiss was estimated and existence of DNA fragments of the wild species in the triticale (6x)
hybrid strains background was confirmed. Seventeen RAPD and fourteen ISSR selected
primers were used in analyses. A total number of 152 bands RAPD and 220 ISSR products
were obtained, out of which 34% were polymorphic in the both cases. In hybrids fourteen RAPD
and twenty ISSR fragments specific for Aegilops crassa (4x) Boiss were identified (9% in the
both cases). Both methods allowed to confirm the hybrid character of all the strains estimated.
Based on the polymorphism of RAPD and ISSR markers, the values of Dice genetic similarity
indices between the pairs of genotypes studied were calculated. Mean genetic similarity
between the hybrid forms and triticale parental components was the same for both methods
used – 0,88. Most different from the others was the maternal species Aegilops crassa 4x Boiss.
Dendrograms showing relatedness between analyzed populations were constructed based on
Dice’s similarities.
Opis
Słowa kluczowe
Aegilops crassa 4x Boiss, ISSR, markery molekularne, podobieństwo genetyczne, pszenżyto, RAPD, genetic similarity, molecular markers, triticale
Cytowanie
Grądzielewska A., Gruszecka D., Paczos-Grzęda E. (2010). Zastosowanie metod RAPD i ISSR do oceny mieszańców pszenżyta z Aegilops crassa 4x Boiss. Folia Pomer. Univ. Technol. Stetin. 2010, Agric., Aliment., Pisc., Zootech. 276 (13), 19–30. https://hdl.handle.net/20.500.12539/1010