QTL Analysis of Chlorophyll Content and Chlorophyll Fluorescence Parameter in Mapping Population of Rye
Ładowanie...
Pliki
Data
2014
Tytuł czasopisma
ISSN czasopisma
Tytuł tomu
Wydawca
Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie
Abstrakt
Zdefiniowano QTL dla zawartości chlorofilu (Chc) i maksymalnej fotochemicznej
aktywności PSII (Fv/Fm) żyta. Materiał do badań stanowiła populacja RIL mieszańca
międzyliniowego DS2 × RXL10, złożona z 70 osobników pokolenia F7. Otrzymane wyniki
poddano analizie statystycznej; stwierdzono istotne zróżnicowanie obu cech u form
rodzicielskich i osobników w populacji mapującej. Nie zaobserwowano istotnej statystycznie
korelacji pomiędzy Chc a Fv/Fm; obliczone współczynniki odziedziczalności w szerokim zakresie
(HB) wynosiły odpowiednio 56 i 53%. Przy LOD ≥ 2,0 wyznaczono 19 regionów QTL, w tym
9 dla zawartości chlorofilu i 10 dla całkowitej wydajności fotochemicznej PSII. Lokalizowały się
one głównie w dystalnych lub centromerowych obszarach chromosomów: 1R, 3R, 5R, 6R, 7R,
przy czym na chromosomach 1R, 5R i 6R znalazły się regiony wspólne dla QTL Fv/Fm1
i Fv/Fm5 (1R), Chc8 i Fv/Fm9 (5R) oraz Fv/Fm3 i Fv/Fm10 (6R). Otrzymane QTL dostarczają
wstępnej wiedzy o dziedzicznym podłożu zawartości chlorofilu w życie i maksymalnej
fotochemicznej aktywności PSII.
The objective of the study was to identify QTLs for the chlorophyll content (Chc) and the maximum photochemical activity of PSII (Fv/Fm) in rye. RIL population of DS2 × RXL10 hybrid cross consisting of 70 individuals of F7 generation constituted as experimental material. The obtained results were subjected to statistical analysis, and significant differences in the diversity of both traits in parental phenotypes and individuals of mapping population, were shown. No statistically significant correlation between Chc and Fv/Fm was observed, and calculated broad sense heritabilities (HB) were estimated at 56 and 53% respectively. With LOD score ≥ 2.0, 19 QTL regions were established, including 9 for the content of chlorophyll and 10 for a total photochemical efficiency of PSII. They were mainly localized in the distal or centromeric regions of 1R, 3R, 5R, 6R, 7R chromosomes. On 1R, 5R and 6R common regions for QTLs were found: Fv/Fm1 and Fv/Fm5 (1R), Chc8 and Fv/Fm9 (5R) as well as Fv/Fm3 and Fv/Fm10 (6R). The resulting QTLs provide preliminary knowledge on hereditary basis of chlorophyll content and maximum photochemical activity of PSII in rye.
The objective of the study was to identify QTLs for the chlorophyll content (Chc) and the maximum photochemical activity of PSII (Fv/Fm) in rye. RIL population of DS2 × RXL10 hybrid cross consisting of 70 individuals of F7 generation constituted as experimental material. The obtained results were subjected to statistical analysis, and significant differences in the diversity of both traits in parental phenotypes and individuals of mapping population, were shown. No statistically significant correlation between Chc and Fv/Fm was observed, and calculated broad sense heritabilities (HB) were estimated at 56 and 53% respectively. With LOD score ≥ 2.0, 19 QTL regions were established, including 9 for the content of chlorophyll and 10 for a total photochemical efficiency of PSII. They were mainly localized in the distal or centromeric regions of 1R, 3R, 5R, 6R, 7R chromosomes. On 1R, 5R and 6R common regions for QTLs were found: Fv/Fm1 and Fv/Fm5 (1R), Chc8 and Fv/Fm9 (5R) as well as Fv/Fm3 and Fv/Fm10 (6R). The resulting QTLs provide preliminary knowledge on hereditary basis of chlorophyll content and maximum photochemical activity of PSII in rye.
Opis
Słowa kluczowe
chlorophyll content, chlorophyll fluorescence, genetic mapping, rye, QTL, fluorescencja chlorofilu, mapowanie genetyczne, zawartość chlorofilu, żyto, QTL
Cytowanie
Molik K., Pawłowska E., Kantarek Z., Milczarski P. (2014). QTL Analysis of Chlorophyll Content and Chlorophyll Fluorescence Parameter in Mapping Population of Rye. Folia Pomer. Univ. Technol. Stetin., Agric., Aliment., Pisc., Zootech. 312(31), 105–116.