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Pozycja Open Access Evaluation of toxicity of biologically synthesized silver nanoparticles (AG-NPs) using Lemna test and Algaltoxkit F(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Chojniak, Joanna; Drobniewska, Agata; Jałowiecki, Łukasz; Biedroń, Izabela; Płaza, Grażyna; Nałęcz-Jawecki, Grzegorz; Microbiology Unit, Institute for Ecology of Industrial Areas, Katowice; Department of Environmental Health Sciences, Medical University of Warsaw; Microbiology Unit, Institute for Ecology of Industrial Areas, Katowice; Microbiology Unit, Institute for Ecology of Industrial Areas, Katowice; Microbiology Unit, Institute for Ecology of Industrial Areas, Katowice; Department of Environmental Health Sciences, Medical University of WarsawIn the current study the toxicity of biologically synthesized Ag-NPs to Selenastrum capricomutum and Lemna minor was assessed. The supernatants from the Bacillus subtilis cultures were applied for the Ag-NPs synthesis. The following media were used for culturing of Bacillus subtillis (I’-1a): molasses (Mol.), brewery effluent (# 6) and Luria-Bertani (LB) medium. Silver nitrate was added to the culture supernatants to the final concentration of 1 mM. The Ag-NPs presence was monitored by UV-Vis spectrophotometer. Toxicity tests were made according to OECD Guideline 201 (2006) and ISO standard 20079 (2006). In the test AlgaltoxkitF the two parameters cell growth and content of chlorophyll a were evaluated. However, in the test with Lemna minor the five endpoints including: number and total area of fronds, fresh weight (f.w.), chlorophylls a (Chl a) and b (Chl b) were used to assess phytotoxicity. Ag+ ions were considered to be the most toxic for Selenastrum capricornutum and Lemna minor than Ag-NPs biologically synthesized. The toxicity of biologically synthesized Ag- NPs was lower to S. capricomutum and to L. minor for all analyzed parameters compared to the toxicity of Ag+. However, the toxicity of Ag-NPs synthesized in the supernatant from the culture of Bacillus subtilis growing on brewery waste was higher compared to the Ag-NPs synthesized in the supernatants from the cultures of strain growing on molasses and LB media. The variability in sensitivity of both organisms towards Ag-NPs was observed.Pozycja Open Access Hydroelectric power plants in the basin of Słupia river – Touristic attraction or ecological threat?(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Bigus, Katarzyna; Astel, Aleksander; Antonowicz, Józef; Nałęcz-Jawecki, Grzegorz; Drobniewska, Agata; Department of Environmental Chemistry, Pomeranian University in Słupsk; Department of Environmental Chemistry, Pomeranian University in Słupsk; Department of Environmental Chemistry, Pomeranian University in Słupsk; Department of Environmental Research, Medical University of Warsaw; Department of Environmental Research, Medical University of WarsawAn abundance of ions and selected heavy metals in subsurface and near-bottom water samples as well as bottom sediments collected above and below of six hydroelectric power plants in the Słupia River basin was assessed together with their toxicity. Except some nutrients, ionic profile of river water indicates its high purity. None of toxicity was found for water samples using Microtox screening, Spirotox and Daphtoxkit F magna tests. Despite the fact that an average concentration of metals in water and bottom sediments was quite low there were some slight toxicity changes observed for sediments. Only in case of two hydroelectric power plants (Krzynia and Skarszów Dolny) sediments collected above were much toxic than these collected below. Observed changes are probably connected with natural or artificial origin of the supplying canals. However, generally hydroelectric power plants do not contribute to significant increase of heavy metals concentration as well as toxicity of both water and sediment samples. Nowadays, they play mainly as extraordinary touristic attraction enabling admiring beautiful architecture and principle of operation.