Przeglądaj wg Autor "Zdrowowicz, Ewa"

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    PozycjaOpen Access
    The Role of Staining Techniques in Seminological Analysis of Mammalian Semen
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2015) Banaszewska, Dorota; Andraszek, Katarzyna; Zdrowowicz, Ewa; Czubaszek, Magdalena; Walczak-Jędrzejowska, Renata; Institute of Bioengineering and Animal Breeding, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland; Institute of Bioengineering and Animal Breeding, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland; Institute of Bioengineering and Animal Breeding, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland; Institute of Bioengineering and Animal Breeding, Siedlce University of Natural Sciences and Humanities, Siedlce, Poland; Department of Andrology and Reproductive Endocrinology, Medical University in Łódź, Poland
    The most important semen parameters are the concentration, motility and morphology of sperm cells. Sperm morphology is regarded as the most reliable parameter for predicting fertility in males. A problem in evaluating sperm morphology and morphometry is the lack of standardization of staining techniques. The staining procedure and reagents used can significantly affect the morphometric parameters of the sperm cell. The use of stains with different pH or osmotic pressure, as well as the duration of the procedure, may influence the shape and size of the sperm, and thus the result of the morphological evaluation of the semen. It is necessary to develop an evaluation procedure for sperm morphology and morphometry that will minimize the changes in the structure of the evaluated semen in relation to the native semen.