Artykuły naukowe (WBiHZ)

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    Renal expression of aquaporin 2 (AQP2) of growing piglets fed diet supplemented with inulin and probiotics
    (Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Michałek, Katarzyna; Kolasa-Wołosiuk, Agnieszka; Staśkiewicz, Łukasz; Department of Physiology, Cytobiology and Proteomics, West Pomeranian University of Technology Szczecin; Department of Histology and Embryology, Pomeranian Medical University, Szczecin; The Kielanowski Institute of Animal Physiology and Nutrition, Polish Academy of Sciences Jabłonna
    Feeds supplemented with probiotics and inulin, which help in the maintenance of desired intestinal microbiota, have beneficial, multidirectional, health-promoting effects. One such effect involves increased bioavailability and absorption of macro- and micronutrients. Enhanced intestinal absorption of many minerals and, in consequence, their elevated blood plasma concentration should provoke a homeostatic response of the kidneys in order to regulate water excretion. Therefore, we have undertaken a pilot study to test the hypothesis that probiotics and inulin added to feed change the localization and increase the expression of aquaporin 2 (AQP2), a protein essential in renal water reabsorption. The study was carried out on 16 Danbred x Duroc crossbred piglets (males). Based on immunohistochemistry (IHC) results, we found that the abundance of AQP2 in animals fed with the modified diet increased mainly in the apical plasma membrane of the collecting duct principal cells. Western blot analysis revealed that in animals fed the supplemented diet the total AQP2 expression in the renal medulla increased too. These changes in the location and expression of AQP2 imply increased renal water reabsorption. Such increased water reabsorption in response to the positive balance of many components seems to support the evidence of the positive effects of dietary probiotics and inulin.