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Pozycja Open Access Alleviating Effects of Ascorbic Acid on Lead Toxicity in Goji (Lycium barbarum L.) in Vitro(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Krupa-Małkiewicz, Marcelina; Kruczek, Arleta; Pelc, Justyna; Smolik, Beata; Ochmian, Ireneusz; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland; Department of Horticulture, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, Szczecin, Poland; Department of Horticulture, West Pomeranian University of Technology, Szczecin, PolandLead (Pb) is the most common heavy metal pollutant in the environment. The objective of the presented study was to investigate the ameliorative effect of exogenous 1 mM ASA on key growth and biochemical parameters in Lycium barbarum seedlings under heavy metal (Pb(NO3)2) stress in vitro. Nodal cutting with an axillaries bud were used as an explants. The results showed that lead accumulation in goji explants had negative influence on morphological parameters of plant growth, such as shoot and root length. Lead caused a significant reduction in chlorophylls and carotenoid content, increased lipid peroxidation and induced significant accumulation of proline in goji leaves. Addition to MS medium 1 mM ASA greatly alleviated Pb-induced growth inhibition and Pb-induced MDA and proline accumulation. Presence of ASA in the MS medium under heavy metal stress increased plant fresh and dry mass with no significant effect on plant water content.Pozycja Open Access Analysis of changes in water management light soil following application of different tillage systems and forecrops(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Schiller, Marta; Podsiadło, Cezary; Stankowski, Sławomir; Pużyński, Stanisław; Michalska, Bożena; Department of Agronomy, West Pomeranian University of Technology, Szczecin,; Department of Agronomy, West Pomeranian University of Technology, Szczecin,; Department of Agronomy, West Pomeranian University of Technology, Szczecin,; Department of Agronomy, West Pomeranian University of Technology, Szczecin,; Department of Meteorology and Shaping Landscape Architecture, West Pomeranian University of Technology, SzczecinThe study was conducted in a static experiment established in 1993 in the Agricultural Experimental Station in Lipnik (near Stargard Szczeciński). Bi-factorial field experiment was set up in the perpendicular bands pattern of randomized sub-blocks in four replicates. Studied factor consisted of the farming systems used for winter wheat after different forecrops (faba beans, sugar beet). Analysed physical properties, shaping water management in light soil, changed depending on the previous crop grown and the amount of rainfall in the growing season. In years with larger rainfall deficits, the soil in the position after faba bean was characterized by a higher density, current humidity, and non-capillary porosity. It referred to the aeration porosity in the position after sugar beet. During years with less rainfall deficiency, the bulk density was increased after sugar beet, while non-capillary porosity decreased after faba bean. Giving up the plowing sowing for the benefit of plowless system or direct sowing modifies some physical properties of topsoils. There was an increase in bulk density and non-capillary porosity. Higher values of bulk density of the soil, listed on the objects, where the applied technologies without plowing, especially on light soils may promote the growth and development of plants commodities, including winter wheat.Pozycja Open Access Analysis of fluoride, dry matter contents and pH in sediments and surface water of Nowogardzkie lake(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Szydłowski, Kamil; Stręk, Michał; Department of Ecology, Environmental Protection and Development, West Pomeranian University of Technology, Szczecin; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, SzczecinThe aim of study was to determine the impact of adjacent terrain usage on the fluoride content in sediments and others important ecological indicators. All of results applies to bottom sediments and surface water from Nowogardzkie Lake. The lake is partly within the Nowogard agglomeration and partly within farmlands. The total number of collected samples was 19 from sediments and 5 from surface water. The all samples were collected in January 2016. Higher concentrations of fluoride and organic matter content were recorded in agricultural adjacent area and pH of sediments was higher in city area. There was correlation between fluoride content in sediments and organic matter content. There was also negative correlation betwen pH in sediments and organic matter content.Pozycja Open Access Analysis of influence farming systems on chemical composition of four variety of triticale winter (xTriticosecale Wittm. ex A. Camus) grain(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Gibczyńska, Marzena; Dawidowski, Andrzej; Sobolewska, Magdalena; Jaroszewska, Anna; Lewandowska, Leokadia; Department of General and Ecological Chemistry, West Pomeranian University of Technology, Szczecin; Grupa Azoty Zakłady Chemiczne „Police” SA, Police; Department of Agronomy, West Pomeranian University of Technology, Szczecin; Department of Agronomy, West Pomeranian University of Technology, Szczecin; Department of General and Ecological Chemistry, West Pomeranian University of Technology, SzczecinThe aim of the study carried out on the basis of a field experiment was to compare results obtained during cultivation of winter triticale in the organic and conventional farming systems. Comparing these two systems (organic with conventional), changes in the content of macroelements (phosphorus, potassium, calcium, magnesium), and microelements (manganese, iron, zinc and copper) were analyzed in the triticale grain. The study involved four triticale varieties: Benetto, Cultivo, Grenado, and Moderato. The field experiment was performed in 2010–2011 at The National Research Institute for Fisheries Affairs Mecklenburg – Vorpommern in Gulzow, Germany. Grain of all triticale varieties grown in an organic systems contained more phosphorus and magnesium. Compared tillage systems did not differentiate calcium and potassium in grain of triticale Benetto, Cultivo, Grenado, and Moderato varieties. No relationship was found between the total content of phosphorus, calcium and magnesium vs. cultivars. The total potassium content in triticale grain was differentiated by the varietal factor. The triticale grain of all tested varieties (Benetto, Cultivo, Grenado, and Moderato) grown in organic system was characterized by higher – by ten or so per cents – iron, zinc, manganese, and copper contents as compared to the conventional systems.Pozycja Open Access Analysis of macronutrients content in winter wheat grain of spelt type (Triticum aestivum ssp spelta L.) depending on crop protection, seeding standards and variety(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Gawęda, Dorota; Haliniarz, Małgorzata; Hury, Grzegorz; Gibczyńska, Marzena; Department of Herbology and Plant Cultivation Techniques, University of Life Sciences in Lublin; Department of Herbology and Plant Cultivation Techniques, University of Life Sciences in Lublin; Department of Agronomy, West Pomeranian University of Technology, Szczecin; Department of Chemistry, Microbiology and Environmental Biotechnology, West Pomeranian University of Technology, SzczecinThe aim of the study was to evaluate the effect of crop protection and seeding ratio on the contents of macronutrients (nitrogen, phosphorus, potassium, calcium, magnesium) in grain of two spelt cultivars: Polish ‘Rokosz’ and German ‘Schwabenspelz’. The field experiment was conducted in Czesławice in 2014–2015 on lessive soil developed from loess classified as good wheat complex and II bonitation class, slightly acidic. The plowing layer of the soil was characterized by a high content of available forms of phosphorus, potassium and average magnesium. Material for analysis consisted of winter spelt grain. The first experimental factor: winter spelt varieties; second factor: two seeding ratios; third factor: crop protection. The harvest was performed at full grain maturity stage. Content of three basic macronutrients in spelt grain: nitrogen, phosphorus and potassium, was varied for cultivars. Meanwhile two cultivars did not differ with the amount of magnesium and calcium in grain. ‘Schwabenspelz’ was more abundant than ‘Rokosz’ regarding to studied macronutrients. The increase of plant density resulted in significant reduction in the nitrogen content and increase in the amount of phosphorus in winter spelt grain. The amount of plant density did not affect the abundance of spelt grain in relation to metals included in macronutrients occurring at plants. The crop protection treatments of winter spelt did not impact on the macronutrients content in grain.Pozycja Open Access Analysis of the variability of registered cases of lyme disease among workers of private agriculture in west pomeranian voivodship(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Stawicki, Tomasz; Department of Engineering the Agrotechnic Systems, West Pomeranian University of Technology, SzczecinThe analysis of incidence to Lyme disease (Borreliosis) in West Pomeranian Voivodship within 2005–2014, in particular with taking into consideration the cases of Lyme disease diagnosed as an occupational disease among the persons providing work in individual agriculture, has been executed pursuant to the epidemiological data obtained from the sanitary stations. In total, 46 cases of Lyme disease were found among individual farmers within the examination period and they were considered as an occupational pathology in the form of an administrative decision. The distribution of the number of cases of diagnosed occupational disease was characterised by the growing trend at the average growth of the coefficient of incidence to Lyme disease per year - 2.38 cases to one hundred thousand of the persons working in agriculture. The results obtained indicate the growing risk of occurrence of Lyme disease among the persons working in agriculture, however the interpretation of the estimated measures of exposure is not clear. It results from the fact that observed dynamics of the changes of epidemiological status may result from a real growing of the occupational exposure in agriculture (e.g. in the aftermath of environmental changes) and, which is more probable, from a cumulative synergy of the environmental factors, growing awareness of farmers and more effective medical diagnostics.Pozycja Open Access Assessment of agro-meteorological conditions of the vegetation period in 2015 in the Szczecin lowland(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Rawicki, Kacper; Mąkosza, Agnieszka; Institute of Technology and Life Sciences, West Pomeranian Research Centre in Szczecin; Department of Meteorology and Green Areas Management, West Pomeranian University of Technology, SzczecinThe present study presents the analysis of thermal and pluviometric conditions on the basis of selected agro-meteorological indices in the vegetation period (IV–X) in 2015 in the Szczecin Lowland against the reference period of 1961–2014. With the use of the results of automatic measurements of air temperature, the following were identified: the beginning, the end and duration of vegetation period, as well as the sum of effective temperatures above the 5oC threshold. Additionally, the months were thermally classified according to Lorenc (2000) and the pluviometric conditions were assessed with the use of standardised precipitation index (SPI). It was found that in 2015 in the Szczecin Lowland, the thermal vegetation period lasted for 257 days with the date of beginning 19th of March, and the ending date 30th November. Therefore, the period was by 26 days longer than the average and fourth in terms of length as compared with the reference multi-year period. As compared to thermal conditions till the second half of the 1990s, the vegetation period under analysis was marked by high heat resources – the resources were average in comparison to the last several years. However, according to thermal classification, the period was characterised by average thermal conditions, yet the shortage of precipitation resulted in severe atmospheric drought as defined by the values of SPI index. The most unfavorable weather conditions were recorded in August which was marked by abnormally warm thermal conditions and extreme atmospheric drought.Pozycja Open Access Assessment of Solar Radiation in the Region of Lake Miedwie for Recreational Purposes(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Kozmiński, Czesław; Michalska, Bożena; Department of Tourism, University of Szczecin, Szczecin, Poland; Department of Meteorology and Landscape Architecture, West Pomeranian University of Technology, Szczecin, PolandThe aim of the paper is the assessment of the amount and variability of sums of global solar radiation, diffuse radiation and direct radiation in the area of Lake Miedwie and the resulting conditions for recreation. For this purpose, the study was based on hourly and 24-hour period measurements of the aforementioned types of radiation obtained from the Agrometeorological Station in Lipnik in the vicinity of Stargard in the period 2008–2017 – the measurements were taken using Delta T BF3 Sunshine Sensors. The temporal distribution of global, diffuse and direct radiation was determined according to hours, days, months, seasons and years with average and extreme values as well as coefficient of variation (W%). The percentage share of sums of diffuse and direct radiation in global solar radiation was calculated and, on the basis of skin erythema dose (SED), safe tanning time for people with blond, chestnut and dark hair was identified for a given day during the warm half-year in the region of Lake Miedwie. There is a high temporal variability and uneven distribution of the types of radiation observed from month to month as well as from year to year. Moreover, the predominance of direct radiation in spring, as compared with autumn, is more than two-fold and more than 18 times greater in summer than in winter. The course of annual radiation is characterised by the occurrence of several days-long periods of increased or reduced radiation sum. From the last days of March to the first decade of August, mean daily global solar radiation sums is above 4000 W · m–2, and from mid-April to the first days of August it exceeds 5000 W · m–2. From May to August the intensity of direct solar radiation around the noon on sunny days can be onerous and, with increasing sunbathing time, even hazardous to health.Pozycja Open Access Assessment of the progress of integrated pest management products implementation into agricultural production space(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Zatoń, Kinga; Błaszak, Magdalena; Department of Chemistry, Microbiology and Environmental Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Chemistry, Microbiology and Environmental Biotechnology, West Pomeranian University of Technology, SzczecinThe study was aimed at assessing the progress of integrated pest management (IPM) implementation into agricultural practices. Assessment of farmers’ attitude to the new EU policy to reduce the use of insecticides as well as evaluating the real possibilities of biological methods use for plant protection was analyzed. Almost 60% of farmers whose farms were conducted in a conventional system declared the use of pesticides prophylactically. Farmers conducted intensive agriculture do not know the principles of IPM (prohibition of pesticides abuse). However, it is possible that these farmers know the restrictions introduced in 2014 without accepting them. It has been observed that in the group of farmers declaring the knowledge upon principles of IPM, the proportion of respondents aware of pesticides ecotoxicity increases. Therefore, a continuous education of farmers seems justified, but at simultaneous expanding the range of biological products and support mechanisms for farmers, who quit the intensive use of chemicals.Pozycja Open Access Cane Pruning IntensityoOf Vine as a Substantial Factor Influencing Physico-Chemical Attributes of Berries Cultivar ‘Regent’(Wydawnictwo Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Pachnowska, Kamila; Ochmian, Ireneusz; Department of Horticulture, West Pomeranian University of Technology Szczecin, Poland; Department of Horticulture, West Pomeranian University of Technology Szczecin, PolandThe research were focused on the assessment of quality attributes of berries cultivar ‘Regent’. The vines trained on Single Guyot training system were pruned at three intensities of 4, 6 or 8 buds per cane. Berries were harvested in three consecutive seasons (2013–2015) at the research station of West Pomeranian University of Technology Szczecin in Poland. ‘Regent’ grapes were analysed in terms of physical and chemical attributes, i.e. weights of clusters and 100 berries, total soluble solids, titratable acidity, pH, polyphenols, nutrients (Ca, Cu, Fe, K, Mg, Mn, N, P, Zn) and heavy metals (Cd, Pb). Our results showed that cane pruning intensity of vine cultivar ‘Regent’ had significant influence on the quality of berries, with the exceptions of cluster weight, titratable acidity and pH. Weight of 100 berries, total soluble solids and most of elements increased as the intensity of pruning increased. In turn, the lightest pruning treatment led to the greatest polyphenol, K, Cu and Fe contents in berries.Pozycja Open Access Colonization of selected rose varieties by pests and pathogens(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Adamska, Iwona; Dzięgielewska, Magdalena; Department of Ecology, Environmental Protection and Development, West Pomeranian University of Technology, Szczecin; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology, SzczecinThe occurrence of pests and microscopic fungi on roses in the “Rożanka” City Garden in Szczecin was investigated in the years 2014–2015. Rosa thea hybrida (varieties ‘Alchymist’, ‘Aspirin Rose’, ‘Blaze Superior’, ‘Carina’, ‘Die Welt’, ‘Fairy’, ‘Flamingo’, ‘Flammentanz’, ‘Gloria Dei’, ‘Goldstern’, ‘Ingrid Bergman’, ‘Mr Lincoln’, ‘Muttertag Orange’, ‘Santana’ and ‘Sommerwind’), R. x centifolia (‘Petit De Holand’ variety) and R. rugosa were selected for analysis. Species composition and colonization degree by pests and by fungi were evaluated. Colonization degree depended on the rose variety, observation date and presence of other biological factors. An antagonism between pests and microscopic fungi was detected. R. ‘Ingrid Bergman’ was a variety least inhabited by pests and fungi.Pozycja Open Access Comparison of growth, bulbs yield and nutrient content of Eucomis autumnalis (Mill.) Chitt., E. bicolor Baker and E. comosa (Houtt.) Wehrh. grown in a greenhouse as pot plants(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Salachna, Piotr; Department of Horticulture, West Pomeranian University of Technology, SzczecinEucomis species are a relatively new pot plants with potential for expansion. The aim of the study was to compare the growth, flowering, bulbs yield and macronutrients content of three species: Eucomis autumnalis, E. bicolor and E. comosa. The experiment was conducted in 2013 and 2014 in a greenhouse. The bulbs were planted in 18 cm diameter pots containing a deacidified peat, supplemented with a fertilizer Hydrocomplex. Of all the species tested, E. autumnalis had the least leaves, flowered earliest and produced the highest number of adventitious bulbs. E. bicolor had the highest number of leaves, produced longer and wider inflorescences and the least number of adventitious bulbs as compared to the other two species. E. comosa was characterized by the largest diameter of adventitious bulbs and the highest potassium content in the leaves. The analysis of the macronutrient composition of three Eucomis species showed that the leaves contained more nitrogen, phosphorus, potassium, magnesium and calcium than the bulbs.Pozycja Open Access Comparison of propagation method in in vitro and in vivo condition of Lonicera caerulea L.(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Krupa-Małkiewicz, Marcelina; Ochmian, Ireneusz; Smolik, Miłosz; Ostrowska, Krystyna Maria; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Horticulture, West Pomeranian University of Technology, Szczecin; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Horticulture, West Pomeranian University of Technology, SzczecinThe aim of the study was to determine the effectiveness of rooting of blue honeysuckle of ‘Wojtek’ cultivar in vitro. Moreover, suitability of different soil substrate for rooting the lignified herbaceous cuttings in vivo was evaluated. The process of disinfection was the decisive factor in the process of preparing the explants for the propagation. Among the disinfection solutions used for sterilization of ‘Wojtek’ microshoots, the best results were obtained after application of 10% NaOCl solution. Initiation and propagation of blue honeysuckle proceed with the highest efficiency in Murashige and Skoog (MS) medium, and no positive effect of BAP cytokine on this process was reported. Proper selection of composition of the medium guaranteed high performance of rooting of explants in vitro – lack of plant growth regulators (PGRs) in MS medium led to a decrease in the percentage of rooted plants. The addition of auxins to MS medium had a stimulating effect on plant height, length, and number of roots. In the greenhouse, four-node lignified cuttings in the medium prepared with perlite and peat were rooted with the highest performance. Rooting of lignified herbaceous cuttings in the substrate prepared with peat and perlite stimulated the development of the root system as well. The worst influence for rooted cuttings was observed in sand.Pozycja Open Access Dart markers linked with genes controlling restoration of male fertility in hybrid rye cultivars with improved pollen shedding(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2017) Stojałowski, Stefan; Hanek, Monika; Orłowska, Marta Orłowska; Sobczyk, Martyna; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin; Department of Plant Genetics, Breeding and Biotechnology, West Pomeranian University of Technology, SzczecinIn this study, we aimed to apply a high-throughput genotyping method to map genes important for the restoration of male fertility in a hybrid cultivar of rye containing the Pampa sterilizing cytoplasm. The diversity arrays technology (DArT) was used to analyse 48 individuals of the F2 population obtained by crossing the male-sterile S305P line with a plant randomly chosen in the Gonello F1 cultivar. In addition to DArT markers, a set of previously published PCR-based markers was also used for genotyping. In total, more than 3300 markers were used in this study. A mapping analysis allowed a construction of seven linkage groups containing 763 markers covering a total distance of approximately 520 cM. Eighty molecular markers were applied to identify genomic regions important for the male fertility restoration. Their distribution indicated the presence of a major and minor restorer genes on chromosomes 4RL and 1R, respectively. These results were consistent with previous reports on the genetic control of the male fertility in the CMS-Pampa. Moreover, new molecular markers located in chromosomal regions significantly associated with the restoration of male fertility were found as well, including PCR-based markers converted from the DArT markers.Pozycja Open Access Effect of calcium peroxide on oxidoreductase activities in soil contaminated with gasoline and diesel oil(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Stręk, Michał; Telesiński, Arkadiusz; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology Szczecin; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology SzczecinThis paper presents the study on assessment of the effect of calcium peroxide on activity of chosen enzymes: dehydrogenases, catalase and o-diphenol oxidase in soil contaminated gasoline or diesel oil. Laboratory experiment was carried out on loamy sang with Corg content 8.7 g ・ kg–1. Into soil samples different combinations of gasoline or diesel oil (at the dosage of 50 g ・ kg–1) and calcium peroxide (at the amounts of 100 and 200 mg ・ kg–1) were added. Samples were adjusted to 60% maximum water holding capacity, and they were incubated for four weeks in tightly closed glass containers at temperature of 20°C. In soil treated with CaO2 and petroleum products, soil oxidoreductase activities were measured on days 1, 7, 14 and 28. Soil contamination with gasoline decreased in all oxidoreductase activities, while contamination with diesel oil increased in dehydrogenases and caused slight changes in catalase and o-diphenol oxidase activity. Effect of CaO2 on oxidoreductase activities in soil uncontaminated and contaminated with gasoline and diesel oil proved diversified, and depended on enzyme, incubation time, type of petroleum product and calcium peroxide dosage. Application of CaO2 in soil contaminated with petroleum products caused mainly stimulation of dehydrogenases and catalase.Pozycja Open Access The effect of different doses of fertilisation with phosphorus, potassium and nitrogen on seed yield of Dactylis glomerata L. of ‘Tukan’ variety(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Kitczak, Teodor; Czyż, Henryk; Kirkiewicz, Anna; Department of Soil Science, Grassland and Environmental Chemistry West Pomeranian University of Technology, Szczecin; Department of Soil Science, Grassland and Environmental Chemistry West Pomeranian University of Technology, Szczecin; Department of Soil Science, Grassland and Environmental Chemistry West Pomeranian University of Technology, SzczecinThe study was conducted at the Lipki Agricultural Experiment Station in Lipnik near Stargard Szczeciński on acid brown soil formed of light glacial sand on clay (5 Bw pgl : pgm), which is characterised by low content of humus on Ap level (1.57–1.59%) and clay particles (11.9–12.4%). The field experiment was set up in split-plot system in four replications, with an area of a single plot of 12 m2. The study included two factors: I – doses of phosphorus and potassium (P + K kg . ha–1): 60 + 120 and 90 + 180, II – doses of nitrogen (N kg . ha–1): 0, 40, 80 and 120. The experiment was established in 2010 by sowing Dactylis glomerata seeds of ‘Tukan’ variety (8 kg . ha–1) in spring barley harvested for grain. The study included two years of seed collection (2011 and 2012). The study determined plant density in the years of full use, number of generative shoots per plant, inflorescence length, number of spikelets and seeds per inflorescence as well as seed and straw yield. Biometric observations were conducted in 25 randomly selected inflorescences from each plot. Seed and straw yield was determined on the area of each plot during seed harvest. Dactylis glomerata of ‘Tukan’ variety was characterised by higher level of yields in the first than in the second year of seed collection. The obtained results indicate, that when establishing a plantation on light soil with companion planting in spring barley, it is advisable to use 120 kg N, 60 kg P and 120 kg K . ha–1. The increased phosphorus-potassium fertilisation (90 kg P and 180 kg . ha–1) did not change the studied morphological features of plants (number of generative shoots per plant, length of inflorescence, number of spikelets and seeds per inflorescence), while fertilising with nitrogen in doses of 40, 80 and 120 kg . ha–1 had a positive effect on increasing the above mentioned features.Pozycja Open Access Effect of Drying Temperature and Method of Extract Preparation on Antioxidant Activity of Edible Flowers of Some Ornamental Plant Species(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2020) Stefaniak, Anna; Grzeszczuk, Monika; Department of Horticulture, West Pomeranian University of Technology in Szczecin, Poland; Department of Horticulture, West Pomeranian University of Technology in Szczecin, PolandDepartment of Horticulture, West Pomeranian University of Technology in Szczecin, Poland Abstract. Edible flowers are a popular food ingredient in many regional cuisines, especially Asian and Middle Eastern ones. Drying is one of the most popular methods of preserving flowers. On the other hand, a common method of consuming dried edible flowers is to use them for preparing various kinds of beverages, both alcoholic and non-alcoholic ones. The paper presents the results of the laboratory analyses concerning the content of biologically active compounds in dried flowers in three temperature variants (~25°C, 35°C and 70°C), which were used to select the material constituting the basis for obtaining various types of water (infusions, decoctions, macerates) and alcohol extracts (with the use of 50%, 80% and 96% ethanol and repeated extractiong method using 96% EtOH), and then their antioxidant activity evaluation. The research material consisted of ornamental plant species with flowers of high biological value: Mimulus × hybridus L. (‘Magic Yellow’ and ‘Magic Red’), Hemerocallis × hybrida Hort., Monarda didyma L., Paeonia lactiflora Pall. (‘Sarah Bernardt’, ‘Dr. Alexander Fleming’ and ‘Karl Rosenfield’). The experiment was carried out in the years 2014–2015 in the Department of Horticulture of the West Pomeranian University of Technology in Szczecin. On the base of the chemical analyses results the most favourable drying temperature variant was selected for each tested flower cultivar. For flowers M. × hybridus L., M. didyma L. and H. × hybrida Hort. it was 35°C; dried flowers were characterised by the highest content of biologically active compounds and the highest antioxidant activity. In the case of three cultivars of P. lactiflora Pall. the most favourable drying temperature was ~25°C. Among the water and alcohol extracts of the studied edible flower species, the decoction indicated a higher content of antioxidant compounds. The highest values of antioxidant activity were observed in extracts prepared on the basis of peony flowers.Pozycja Open Access The Effect of Mannitol and Sorbitol on Soybean In Vitro Development(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Kulpa, Danuta; Gawlik, Andrzej; Matuszak-Slamani, Renata; Włodarczyk, Małgorzata; Bejger, Romualda; Sienkiewicz, Mariola; Gołębiowska, Dorota; Semeniuk, Anna; Department of Plant Genetic, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Physics and Agrophysics, West Pomeranian University of Technology, Szczecin, Poland; Department of Plant Genetic, Breeding and Biotechnology, West Pomeranian University of Technology, Szczecin, PolandThe aim of this study was to examine an effect of osmotic stress, induced by mannitol (Mn) and sorbitol (So) in concentrations (0–200 mM) on seed germination and development of common soybean (Glycine max L.) seedlings in in vitro conditions. The analysis of the effectiveness of the porcession was made by assessing the length of the stems and roots as well as the fresh and dry mass of 3-week-old seedlings. The biometric index values differed depending on the type of substance used. While Mn at the lowest concentration (50 mM) did not affect the biometric parameters studied, So (50 mM) stimulated the growth of seedlings, root elongation and the number of leaves and fresh weight of plants. The unfavorable effect on biomass was noticed at higher concentrations of both substances. Mn (> 50 mM – <150 mM) negatively influenced the fresh and dry content of the seedlings. In turn, Mn (150 and 200 mM) also caused growth inhibition, decreased number and length of leaves. So (200 mM) significantly inhibited the growth of seedlings, reducing the dry matter.Pozycja Open Access The Effect of Multicomponent Mineral Fertilizers on the Content of Macronutrients in Soil After Sugar Beet Cultivation(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2018) Sobolewska, Magdalena; Pużyński, Stanisław; Dojss, Dariusz; Hury, Grzegorz; Gibczyńska, Marzena; Department of Agronomy, West Pomeranian University of Technology, Szczecin, Poland; Department of Agronomy, West Pomeranian University of Technology, Szczecin, Poland; Grupa Azoty Zakłady Chemiczne „Police” S.A., Poland; Department of Agronomy, West Pomeranian University of Technology, Szczecin, PolandA wide range of multicomponent fertilizers, often with similar chemical composition, differs in the quality and digestibility of nutrients and price. The paper compared the effect of mineral multicomponent fertilizers from Polish and foreign producers on changes in the content of macronutrients in soil during sugar beet cultivation. The study also analyzed the effect of varying doses of fertilizers used. Fertilizers used were characterized by the following composition of NPK(S): two Belarusian 8–20–30, Russian 7–8, 19–20, 29–30 and Polish 6–20– 30(7) – Polifoska 6. The experimental plant was sugar beet of NATURA KWS cv., normal-sugar type. The soil on which the experiment was carried out was characterized by low abundance of available potassium and medium available phosphorus and exchangeable magnesium. The experiment was carried out in 2014–2017 in Lipnik. The study compared two factors: 1st factor – 4 multicomponent mineral fertilizers (two Belarusian, one Russian and Polifoska). 2nd factor – 3 doses of fertilization (minimum, optimum, maximum, which amounted to 2.5, 5.0 and 7.5 dt per hectare, respectively. Fertilization with multicomponent fertilizers did not have a significant impact on changes in the pH of experimental soil. In the experiment carried out, there was no difference in the performance of tested multicomponent fertilizers: Belarusian, Russian and Polish, regarding changes in the content of available phosphorus as well as available and exchangeable magnesium in the soil. The use of different doses of multicomponent fertilizers resulted in a significant increase in the content of available potassium and exchangeable magnesium in the soil, and the available phosphorus after using the highest dose of fertilizer.Pozycja Open Access Effect of preharvest use of anti-cracking preparations on changes in selected parameters of sweet cherry fruits during frozen storage(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2016) Telesiński, Arkadiusz; Mikiciuk, Grzegorz; Mikiciuk, Małgorzata; Stręk, Michał; Płatkowski, Maciej; Statkiewicz, Małgorzata; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology Szczecin; Department of Horticulture, West Pomeranian University of Technology, Szczecin; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology Szczecin; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology Szczecin; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology Szczecin; Department of Plant Physiology and Biochemistry, West Pomeranian University of Technology SzczecinThis paper presents the study on comparison of the changes of dry weight content, total polyphenols content, total flavonoids content, and antioxidant activity in sweet cherry fruit in frozen storage, depending on the used preharvest anti-cracking preparations. The study material was sweet cherry fruits of the ‘Burlat’ cultivar, that were treated with anti-cracking preparations. In fresh fruits and during their frozen storage at temperature –25°C (after 60, 120, and 180 days), the mentioned parameters were determined. The preharvest use of anti- -cracking preparations caused an increase in total flavonoids content, and antioxidant activity was observed. In plant untreated anti-cracking preparations, decrease in antioxidant parameters were observed. The preharvest use of during frozen storage deepened the decrease of total flavonoids content and antioxidant activity but limited the changes in the content of dry weight and total polyphenols. The analysis of the kinetic degradation demonstrated that total flavonoid content was characterized by higher stability in the fruits, which were not treated with anti- -cracking preparations. An opposite trend was determined for total polyphenols content.