FPUTS seria Agricultura, Alimentaria, Piscaria et Zootechnica, 2023
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Pozycja Open Access Genetic Aspects of Lactose Synthesis in Dairy Cattle(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2023) Śpiewak , Magdalena; Kowalewska, Inga; Czerniawska-Piątkowska, Ewa; Department of Genetics, West Pomeranian University of Technology, Szczecin, Poland; Department of Genetics, West Pomeranian University of Technology, Szczecin, Poland; Department of Ruminant Science, West Pomeranian University of Technology, Szczecin, PolandMarket requirements, dictated by the growing needs of consumers, make it necessary to conduct breeding works to improve the performance characteristics of farm animals. The effectiveness of the breeding goal depends on both the genotype of the animals and the environmental conditions. Genomic selection using single nucleotide polymorphism (SNP) is increasingly used in the selection and evaluation of dairy cattle breeds. In recent years, many experiments have been carried out to determine the relationship between the occurrence of the genotype and performance traits of livestock. The analysis of milk composition carried out so far focused mainly on such milk production traits as milk yield (kg), fat yield (kg), fat content (%), protein yield (kg) and protein content (%). So far, no largescale experiments have been carried out to test the content of lactose in milk and evaluate possible relationships with other milk performance traits. Lactose synthesis in the epithelial cells of the mammary gland serves as a major factor influencing milk volume production. Due to that conducting such an analysis seems to be beneficial for milk producers for economic reasons. An additional advantage may be the use of the obtained results in marker-assisted selection (MAS). The present review summarizes knowledge about lactose synthesis by covering and linking several aspects of cow’s milk.Pozycja Open Access Polymorphism in BMAP-27 and BMAP-28 genes and their relationship with milk production traits in cattle(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2023) Hiller, Sonia; Kowalewska, Inga; Department of Genetics, West Pomeranian University of Technology, Szczecin, Poland; Department of Genetics, West Pomeranian University of Technology, Szczecin, PolandThe study aimed to analyze polymorphisms located in exon 4 of the CATHL5 gene encoding the BMAP-28 protein and in exon 4 of the CATHL6 gene encoding the BMAP-27 protein concerning milk performance parameters, such as milk yield, fat, protein, and lactose content and somatic cell count in milk. The PCR-RFLP method using the ACRS technique was used in the study to create a cleavage site for enzymes. Based on the results of genotyping, results for individual SNPs were obtained, with statistically significant differences at the P ≤ 0.05 and P ≤ 0.01. Between individual genotypes of the studied polymorphisms and selected traits of milk production.Pozycja Open Access Polymorphism in the GHRL Gene and Milk Production Traitsin Jersey Cattle(Wydawnictwo Uczelniane Zachodniopomorskiego Uniwersytetu Technologicznego w Szczecinie, 2023) Śpiewak, Magdalena; Kowalewska, Inga; Department of Genetics, Faculty of Biotechnology and Animal Breeding, West Pomeranian University of Technology, Szczecin, Poland; Department of Genetics, Faculty of Biotechnology and Animal Breeding, West Pomeranian University of Technology, Szczecin, PolandThe aim of this study was to assess potential relationships between GHRL 4491A˃G genotypes and selected milk production traits, including milk yield, protein and fat content, and protein and fat yield. The study involved 180 Jersey cows. Genotypes of individual animals were determined using the ACRS and PCR-RFLP methods. The allele frequencies were as follows: A – 0.98 and G – 0.02. The conducted research revealed statistically significant relationships (P ≤ 0.05) between GHRL genotypes and milk protein content, and tendencies were observed regarding the association of genotypeswith analyzed milk production traits.